Webb1 feb. 2003 · The samples before and immediately after exposure were all maintained in a water bath at 37.0 ± 0.1°C (Thermo Minder Ex, Taitec). After the treatment procedure, 200 μL of the sample was taken out for a viability test and the remaining cells in each sample were collected by centrifugation at 270 × g for 3 min, and then 3 mL of a fresh medium, … Webbsonal-11 and THERMO MINDER EX, Taitec Corporation) at 37 C for 20 min. Following pre-incubation, the mixture was added and mixed with 2 mL of top agar containing 0.05 mmol/L of L-histidine and 0.05 mmol/L D-biotin and spread over each minimum glucose agar plate. The top agar subsequently was solidified on a flat sur-

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