WebEquation (2) gives the paracellular flux (J) for fluorescein, with ∆t being the duration of the measurement period, and A being the area within the Ussing chamber. Finally, the paracellular permeability can be calculated by using Equation (3), with c a being the fluorescein concentration in the apical side of the Ussing chamber. Results were ... WebJan 1, 2015 · The Ussing chamber system is named after the Danish zoologist Hans Ussing, who invented the device in the 1950s to measure the short-circuit current as an indicator …
The Ussing type chamber model to study the intestinal ... - PubMed
WebJul 31, 2024 · A six Ussing chamber system was used for the intestinal transport experiments (Physiologic Instruments; San Diego, CA, USA). The Ussing chambers were set up on a heating block connected to a VCC MC6 MultiChannel voltage–current clamp via six input modules, containing integral dummy membranes. WebUssing chamber assays use electrodes to measure ionmovement across the membranes of cells grown into a monolayer with tight junctions. "Patch clamp" assays measure the opening and closing rates of single channels, in which patches of the cell membrane are isolated using a micropipette tip and these patches are hooked up to microelectrodes. switch timers for lights
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WebNaviCyte Ussing Chamber (Vertical, Std, 9mm, Round) WI-660014. A standard volume NaviCyte Vertical Ussing Chamber that has a working reservoir volume of 5 to 7 ml. Tissue is affixed between the opposing faces of two half-chambers using a series of pins that surround a 9 mm round aperture (0.64 cm 2 exposed tissue area). WebUssing chambers were initially developed to measure the transport of ions, nutrients, and drugs across various epithelial tissues (Ussing and Zerhan 1951, see Chap. 23). With the … An Ussing chamber is an apparatus for measuring epithelial membrane properties. It can detect and quantify transport and barrier functions of living tissue. The Ussing chamber was invented by the Danish zoologist and physiologist Hans Henriksen Ussing in 1946. The technique is used to measure the short … See more The Ussing chamber provides a system to measure the transport of ions, nutrients, and drugs across various epithelial tissues, (although can generate false-negative results for lipophilic substances ). It consists of two … See more The main types of Ussing chamber systems are: • The Classic chamber system (as designed by Ussing). • The Self-contained chamber system (the full apparatus within a single block) See more • Biography of The Royal Society: Hans Henriksen Ussing (PDF; 1,1 MB) • A guide to Ussing chamber studies of mouse intestine (Am J Physiol Gastrointest Liver Physiol. Jun 2009; 296(6): G1151–G1166. Published online Apr 2, 2009.) See more switch-time