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Sftpc-creert2

WebUsing cell state trajectory modeling, guided by RNA velocity of single cells from 18 timepoints and lineage tracing Sox2-CreERT2 and Sftpc-CreERT2 mice, we show that … Web将IRES-CreERT2敲入到小鼠Sftpc基因3'UTR中,他莫昔芬诱导型Cre重组酶CreERT2受内源Sftpc基因启动子驱动。 诱导后,在II型肺泡细胞中表达Cre活性。 应用领域 :II型肺泡细 …

American Journal of Respiratory Cell and Molecular Biology

Web4 Nov 2024 · SftpcCreERT2/+;tdTomatoflox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, … cheap hotels in honolulu kamaaina https://shift-ltd.com

Metastatic-niche labelling reveals parenchymal cells with stem …

Web12 Jul 2024 · SFTPC creERT2 mice were generated as previously described . To generate the Gli2 creERT2-tdT mouse, a CreERT2-2A-tdTomato expression cassette was inserted … Web22 May 2024 · Sftpc CreERT2/+; tdTomato flox/flox mice were used for the labelling of AT2 cells and labeled subpopulations were analyzed by flow cytometry, qPCR, ATAC-seq, gene … Web3 Mar 2024 · We lineage-labeled AT2 cells using SftpcCreERT2; R26REYFP mice and subjected them to acute lung injury ( Figure 4 D). Ten days after the injury, the lineage … cheap hotels in tuki tuki

Fgfr2b signaling in Alveolar Epithelial type II cells following ...

Category:Age-dependent alveolar epithelial plasticity orchestrates lung ...

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Sftpc-creert2

Early lineage specification defines alveolar epithelial ontogeny in the murine lung …

Web28 Feb 2024 · The SftpccreERT2 mouse line was a gift of H. Chapman; their genotyping and generation have previously been described 34. Hopx3FlagGFP mice 35 were a gift of R. … Web28 Mar 2024 · Mice were maintained under specific pathogen-free conditions. The Kras LSL, Etv5 fl, Trp53 fl, Rosa26 CreERT2, Rosa26 tdTomato, and Sftpc CreERT2 alleles were …

Sftpc-creert2

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Web15 Jun 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell … Web20 Jan 2024 · Quantitatively, AT2 cells from Sftpc CreERT2; Rosa26 miR-302 lungs exhibited increased mitochondrial area and decreased cristae density per mitochondrion, compared …

WebAlveolar type II (AT2) cell dysfunction contributes to a number of significant human pathologies including respiratory distress syndrome, lung adenocarcinoma, and … Web19 Oct 2024 · The Sftpc-CreERT2 mouse line has been used previously to track AT2 cell fate (65, 66). Gramd2-CreERT2 mice were generated by homologous recombination insertion …

Web7 Oct 2024 · To assess the relative contributions of AT2 and AT1 cells to tissue regeneration after hyperoxic injury, we lineage traced AT2 and AT1 cells using the … Web30 Mar 2024 · Next, we assessed whether the integrin-FAK pathway is essential for AT2-AT1 cell differentiation in vivo.We lineage-labeled AT2 cells using Sftpc CreERT2; R26R EYFP …

Web29 Jul 2024 · SftpcCreERT2+/− mice were used as controls. b Genotyping of DNA extracted from CD45 (−)EpCAM (+) cells and CD45 (-)EpCAM (−) cells isolated from control and …

Web028054 B6.129S- Sftpc tm1(cre/ERT2)Blh /J These knock-in Sftpc-CreER T2 mice express a tamoxifen-inducible Cre recombinase from the endogenous promoter/enhancer elements … cheap hotels in sitka alaskaWeb9 Mar 2024 · To specifically confirm which Fgfrs are expressed in mouse AEC2s, we combined the SPC + AEC2-specific Cre recombinase (Sftpc CreERT2) with the ROSA … cheap hotels in uusikaupunkiWebUsing the Sftpc CreERT2; tdTomato lineage-traced mice, we recently reported a novel AT2-IAAP subpopulation characterized by low Sftpc expression but that is enriched for PD-L1 … cheap hotels kailua kona hiWeb20 Mar 2024 · P1 Bacteriophage Cre recomibinase, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA(CreERT2), D6 Bacteriophage rox site, mouse PGK … cheap hotels in yokosuka japanWeb5 May 2024 · Introduction. Idiopathic pulmonary fibrosis (IPF) is a common form of interstitial lung disease resulting in alveolar remodeling and progressive loss of … cheap hotels kailua 2c konaWeb10 Jan 2024 · Lungs from WT, Sftpc CreERT2 R26R EYFP, and Axin2 CreERT2–TdTomato mice were processed into single-cell suspensions as indicated above. WT AT2 cells: AT2 … cheap hotels kauai poipuWeb10 May 2024 · (A) Adult Sftpc CreERT2;R26R EYFP or Hopx CreERT2;R26R EYFP mice were induced with tamoxifen 3 times to label AT2 or AT1 cells, respectively. Following a 2-week … cheap hotels yukon ok