Opti mem thermo
WebSep 30, 2024 · All siRNAs used in this study were obtained from RIBO Biotechnology (China) and transfected into CCA cells using Lipotransfectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the instructions. Briefly, CCA cells were seeded in a six-well plate until the cell density reached approximately 60%. 200 μL serum-free OPTI-MEM ... WebSubsequently, 100 µl Opti-MEM was used to dilute 8 µl Lipofectamine 2000 reagent, which was incubated at room temperature for 5 min. A total of 100 pmol of each construct was diluted with 100 µl Opti-MEM following incubation for 5 min, and then was transfected into cells. All dilutions were performed at room temperature for 10 min.
Opti mem thermo
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WebApr 12, 2024 · For transfection, HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µl Lipofectamine ... WebThermo Fisher opti mem serum free media Opti Mem Serum Free Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
WebJun 18, 2024 · Thermo Fisher Scientific: 10687010: Puromycin: Thermo Fisher Scientific: A1113803: Potassium bicarbonate: Thermo Fisher Scientific: ICN15255780: Enrofloxacin (Baytril) Sigma-Aldrich: ... (25 μg retroviral plasmid and 20 μg pCL-Eco helper plasmid in 1 mL Opti-MEM) and mixture B (add 135 μL 1 mg/mL PEI into 1 mL Opti-MEM). Transfer the …
Webof 100 ng of mRNA in 2.5 microlitres of Opti-MEM (Thermo Fisher Scientific) and 200 ng of the lipofectamine transfection reagent MessengerMAX (Thermo Fisher Scientific) in 2.5 microlitres of Opti-MEM to each well. Fluorescence (Excitation: 485nm; Emission: 528nm) was recorded in real time at 37°C using Cytation™ 3 WebItem Opti-MEM™ I Reduced Serum Medium Company Thermo Fisher Scientific Price Pricing Info Get Quote Get Quote for this Product Catalog Number 31985062 Quantity 100 mL Applications CELL CULTURE Regulatory Status For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals Type Medium
WebGibco™ Opti-MEM I Reduced Serum Media by Thermo Fisher Scientific Manufacturer Thermo Fisher Scientific Model: NULL Available Worldwide 5.0 / 5.0 1 reviews Write your own review An improved MEM formulation that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% Gibco™ Opti-MEM I Reduced Serum Media Request …
WebOpti-MEM and Lipofectamine 2000 were purchased from the Cell Signaling Technology Corporation (Danvers, MA, USA). A549 cells in logarithmic growth phase were seeded in … candidates for malvern electorateWebopti mem ( Thermo Fisher ) Thermo Fisher is a verified supplier Thermo Fisher manufactures this product About News Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. fish pie with creme fraiche recipeWebJan 28, 2024 · Dilute 30 μg of DNA plasmid into 1.5 mL of Opti-MEM Reduced Serum Medium and mix gently. (b) Concomitantly, dilute 80 μL of ExpiFectamine 293 reagent in Opti-MEM Reduced Serum to a total volume of 1.5 mL, mix gently, and incubate at room temperature for 5 min. (c) fish pie with creme fraiche sauceWebProduct name Opti-MEM® I Reduced-Serum Medium (1X), liquid Page 4 / 8 None None Chemical Name Korea OEL (CEILING) 18-Aug-2015 Korea OEL (TWA) Korea OEL (STEL) www.thermofisher.com None Always wear recommended Personal Protective Equipment. Wear personal protective equipment. Product code Ammonium metavanadate 7803-55-6 … candidates for massachusetts senateWebDec 27, 2014 · Because we have 4 siRNAs, we will prepare a master mix of RNAiMAX. Add 200 microliters of Opti-MEM medium and 12 micoliters of RNAiMAX in a tube labelled "Master Mix" Mix well by vortexing or flicking the tube. Add 50 microliters of Opti-MEM Medium into each of 4 tubes and label them 1, 2, Positive, and Negative. candidates for mayor in milwaukeeWebSep 22, 2024 · After 24 h, cells were transfected with 30 μg of pSF-ST2-Fc plasmid DNA diluted in Opti-MEM™ I Medium (Thermo Fisher Scientific, Waltham, MA, USA) … candidates for mayor hamilton ontarioWebOpti-MEM® I Reduced-Serum Medium is a chemically defined, low protein, Minimal Essential Medium (MEM) containing insulin, transferrin, hypoxanthine, thymidine, and … fish pie with eggs recipe uk