WebFITC has excitation max of 490 nm and emission max of 525. 6-FAM can be use to replace FITC. This dye can be directly incorporate at time of synthesis. FITC dye has two mixture of isomers at 5 or 6 position of the ring. Single isomer incorporation is also avaiable. FITC dye can be incorporated at any position within an Oligonucleotide. http://drmr.com/abcon/AxVFITC.html

FITC (Fluorescein 5-isothiocyanate) Fluorescent Dye

WebAn FITC dye has emerged as an excellent choice owing to its excellent pH dependence nature. FITC fluorescence has been shown to increase in pH range 5.0 through 8 ... phase containing PVA and an FITC-GC conjugate to form an emulsion (organic to aqueous phase ratio 1:3). The emulsion was further sonicated for 5 min and stirred at WebDescription. Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow ... ip67 sealing grommet

FITC labeling - Peptideweb.com

WebFITC (fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with … WebColorWheel® Antibody-Ready FITC Dye is designed for use with ColorWheel® Dye-Ready antibodies in Flow cytometry. US EN. Applications Products Services Support. Greener … WebMar 9, 2015 · Anti-RFP Antibody 4 3.3 Calculate the degree of labeling: where 68,000 cm–1M–1 is the molar extinction coefficient of the dye at pH 8.0 at 494 nm. Storing the Conjugates • Store the labeled protein—which will be in PBS with 2 mM sodium azide if the provided spin columns were used—at 4°C, protected from light. • If the final … opening to barney home sweet homes 1993 vhs