WebTaKaRa BCA Protein Assay Kitは、高感度にタンパク質溶液の比色定量を行う試薬であり、界面活性剤によって可溶化されたタンパク質溶液の定量も可能である。 ... WebNote: 2.0 mL of the WR is required for each sample in the test-tube procedure, while only 200 µl of WR reagent is required for each sample in the microplate procedure. 2. Prepare WR by mixing 50 parts of BCA Reagent A with 1 part of BCA Reagent B (50:1, Reagent A:B). For the above example, combine 50 mL of Reagent A with 1mL of Reagent B.
BCA Protein Assay Kit The BCA protein assay is a simple and …
WebFeatures of the Pierce BCA Protein Assay Kit (Reagents A and B) include: • Colorimetric —estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm • Uniformity —exhibits less protein-to-protein variation than dye-binding methods (Bradford) WebBCA Protein Assay Reagent A is a component of the Pierce BCA Protein Assay Kit, a two-component, high-precision, detergent-compatible assay that is used for total protein … baia tejo ementa
[생명과학실험] Protein quantification : BCA assay : 네이버 블로그
Web2. Prepare WR by mixing 25 parts of Micro BCA Reagent MA and 24 parts Reagent MB with 1 part of Reagent MC (25:24:1, Reagent MA:MB:MC). For the above example, combine 12.5mL of Reagent MA and 12.0mL Reagent MB with 0.5mL of Reagent MC. Note: When Reagent MC is initially added to Reagents MA and MB, turbidity occurs that quickly … WebThe BCA Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and primarily relies on two reactions. Firstly, the peptide … WebNote: The volume of BCA working reagent for the test tube assay is based on 1 ml of reagent per reaction. This volume is sufficient if reading the absorbance in a microcuvette (0.7 ml). If the cuvette requires a larger minimum volume, up to 3 ml of reagent may be need per reaction. Table 2 Preparation of BCA working reagent For each sample × 20 aqua kita nürnberg stellenangebote